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KMID : 0370220170610050267
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Yakhak Hoeji
2017 Volume.61 No. 5 p.267 ~ p.273
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Inhibitory effects of coumarins from the fruit of Citrus aurantium against iNOS activity
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Kim Beom-Sun
Jung Eui-Gil
Youn Hyung-Sun
Hwang Kyung-A
Han Man-Deuk
Yu Yon-Ho
Nam Kung-Woo
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Abstract
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Other SectionsAbstractCitrus fruits have been used as an edible fruit, and also as a traditional medicine, since ancient times. A crude methanol extract of Citrus aurantium was shown to suppress inducible proinflammatory cytokines, but its constituents were not known. In this study, we isolated four furanocoumarins, bergapten (1), imperatorin (2), isoimperatorin(3), and phellopterin (4), and one 7-geranyloxycoumarin, auraptene (5), from a methanol extract of dried Citrus aurantium fruit. The structures of these compounds were elucidated by extensive application of spectroscopic techniques, including 2D NMR spectroscopy and mass spectrometry. All of the isolated compounds were evaluated for their ability to inhibit the production of proinflammatory cytokine inducible nitric oxide synthase (iNOS) in lipopolysaccharide-activated RAW264.7 cells. Compounds 2?5 significantly suppressed iNOS production but compound 1 had no iNOS inhibitory effects. These results indicate that at least three furanocoumarins and one 7-geranyloxycoumarin are involved in the suppressive effects of citrus fruits against the production of inducible inflammatory cytokines.
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KEYWORD
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Citrus aurantium, furanocoumarins, 7-geranyloxycoumarin, iNOS, inflammation
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